AI De Novo Protein Platform
The ground-breaking LuxSit™ Pro, de novo binders, and the biosensors that Monod Bio is developing are based on a dramatically expanded development space enabled by our AI-powered de novo protein design platform. Monod Bio is at the forefront of using AI de novo protein design for the development of new research use only (RUO) tools and in vitro diagnostics (IVD) for use in the central lab and point-of-care (POC) settings.
Reporters
LuxSit™ Pro is the world’s first fully de novo luciferase.
- Reduced amino acid dictionary: LuxSit™ Pro has a Cysteine- and Lysine-free sequence to avoid unwanted post-translational modifications
- Highly specific to the Monod Bio proprietary synthetic substrate (minimal cross-reactivity with luciferin, CTZ, DTZ, or FRZ)
- LuxSit Pro can be genetically fused to N or C terminus of the protein of interest. Both plasmids are available for purchase
- Brightness and linear range make it ideal for both RUO and IVD
- Small (13.5 kDa) and hyperstable (Tm above 95°C)
- Visible light emission (peak emission at 490 nm)
- Generates high-intensity glow-type luminescence multiple times brighter than the commonly used Firefly & Renilla luciferase systems
Split LuxSit™ Pro Specifics
- Reversible complementation reporter for protein-protein interactions
- Composed of 2 fragments: a small fragment (~1.5 kDa) and a big fragment (~12 kDa)
- Very low affinity between the 2 components
- Small size and high stability: minimizes interference
Binders
Monod Bio is developing de novo protein binders that provide for sensitive detection of proteins, peptides, and small molecules. These binders enable multiple RUO and IVD applications for high-value targets. These de novo binders are small and highly stable and require no post-translational modifications.
They may also be functionalized with enzymes, fluorescent probes, chemi- or bio-luminescent probes, or site specific surface chemistry.
Biosensors
Monod Bio has created a novel AI/ML protein design platform to create novel protein biosensors. These protein tools are optimal for bioluminescent homogeneous assays that are quantitative, no-wash single-step, fast (<5 min) and use inexpensive reagents.
Commonly available laboratory equipment can be used to quantify the reporter signal.
