LuxSit™ Pro

LuxSit™ Pro

The LuxSit™ Pro Luciferase Assay System generates high-intensity glow-type luminescence multiple times brighter than the commonly used Firefly & Renilla luciferase systems.
The Monod Bio LuxSit™ Pro products are offered as kits that enable scientists to build their desired applications. Each kit provides the following proprietary materials: (1) the reaction buffer, (2) substrate, and (3) substrate reconstitution buffer, and (4) an optional plasmid encoding LuxSit™ Pro.

The LuxSit™ sPro PPI kit is a versatile Two-Component Split Reporter, enabling applications including Protein-Protein interaction (PPI) research and to create novel biosensors.

To purchase, please go to Fisher Scientific at fishersci.com



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LuxSit™ Pro Details

• 
  Reduced amino acid dictionary: LuxSit™ Pro has a Cysteine- and Lysine-free sequence to avoid unwanted post-translational modifications
• 
  Highly specific to the Monod Bio proprietary synthetic substrate (minimal cross-reactivity with luciferin, CTZ, DTZ, or FRZ)
• 
  LuxSit Pro can be genetically fused to N or C terminus of the protein of interest. Both plasmids are available for purchase

• 
  Brightness and linear range make it ideal for both RUO and IVD

• 
  Small (13.5 kDa) and hyperstable (Tm above 95°C)

• 
  Visible light emission (peak emission at 490 nm)

• 
  Generates high-intensity glow-type luminescence multiple times brighter than the commonly used Firefly & Renilla luciferase systems

LuxSit Pro Instructions for Use and Technical Data

LuxSit™ sPro Specifics

• 
  Reversible complementation reporter for protein-protein interactions
• 
  Composed of 2 fragments: a small fragment (~1.5 kDa) and a big fragment (~12 kDa)
• 
  Very low affinity between the 2 components
• 
  Small size and high stability: minimizes interference

LuxSit sPro Instructions for Use and Technical Data

LuxSit™ Pro Kits

Product Name

Size

Price

Kit Features

Product Name

LuxSit™ Pro Luciferase Assay System

Size

1000 reactions

$925

Kit Features

• Small, hyper stable de novo luciferase
• Bright and linear dynamic range
• Highly specific proprietary synthetic substrate
• Peak light emission at 500nm

Product Name

LuxSit™ sPro Protein:Protein Interaction System

Size

1000 reactions

$925

Kit Features

• Split LuxSit™ Pro Luciferase System
• Protein:Protein Interaction System
• Low assay background
• High signal-to-noise upon split enzyme reconstitution

Plasmids

Description

Price

Promoter

Multiple Cloning Site

N- or C- terminal

Size

Format

Description

LuxSit™ Pro, N-term

$250

Promoter

CMV

Multiple Cloning Site

Yes (BamHI, XhoI, SacI, EcoRI, EcoRV, BstXI, XbaI)

N- or C-Terminal

N

Size

25 µg

Format

Lyophi­lized

Description

LuxSit™ Pro, C-term

$250

Promoter

CMV

Multiple Cloning Site

Yes (EcoRV, BstXI, XbaI, SacI, EcoRI, XhoI)

N- or C-Terminal

C

Size

25 µg

Format

Lyophi­lized

Description

LuxSit™ Pro Control

$150

Promoter

CMV

Multiple Cloning Site

No

N- or C-Terminal

n/a

Size

25 µg

Format

Lyophi­lized

Description

ßit sPro, N-term

$125

Promoter

CMV

Multiple Cloning Site

Yes (BamHI, XhoI, SacI, EcoRI, EcoRV, BstXI, XbaI)

N- or C-Terminal

N

Size

25 µg

Format

Lyophi­lized

Description

ßit sPro, C-term

$125

Promoter

CMV

Multiple Cloning Site

Yes (EcoRV, BstXI, XbaI, SacI, EcoRI, XhoI)

N- or C-Terminal

C

Size

25 µg

Format

Lyophi­lized

Description

αLux sPro, N-term

$125

Promoter

CMV

Multiple Cloning Site

Yes (BamHI, XhoI, SacI, EcoRI, EcoRV, BstXI, XbaI)

N- or C-Terminal

N

Size

25 µg

Format

Lyophi­lized

Description

αLux sPro, C-term

$125

Promoter

CMV

Multiple Cloning Site

Yes (EcoRV, BstXI, XbaI, SacI, EcoRI, XhoI)

N- or C-Terminal

C

Size

25 µg

Format

Lyophi­lized

Description

PPI Control Set (FRB:FKBP)

$150

Promoter

CMV

Multiple Cloning Site

No

N- or C-Terminal

n/a

Size

25 µg

Format

Lyophi­lized