LuxLink: Rapid Conjugation System for Custom NovoLISA
Monod Bio’s NovoLISA is a rapid, no-wash (homogeneous) immunoassay powered by state-of-the-art Split LuxSit™ (LuxSit™ sPro) Luciferase technology. The system utilizes two small fragments— βit (~1.5 kDa) and αLux (~12 kDa)—that function as a highly efficient complementation reporter.
These fragments are easily conjugated to antibodies using a standard amine-reactive protocol. When two antibodies bind to separate epitopes on a target analyte, the fragments reconstitute into a functional enzyme. Upon adding LuxSit™ Pro Substrate, the system generates a luminescent signal directly proportional to the analyte concentration.
Optimized for precision, the LuxSit™ sPro system features small, high-stability fragments with a low intrinsic affinity (~89 µM). This design ensures minimal interference with protein binding and delivers fast, reliable results for sophisticated analyte detection.
Key features of LuxLink for NovoLISA
Quick Assay Set Up: Conjugation of LuxSit sPro to the Antibody pair takes less than four hours from beginning to end.
Homogenous “Mix-and-Read”: assay Simplified immunoassay platform that eliminates repeated wash steps and multistep workflows common in conventional ELISAs.
Minimal Interference: The small, lysine-free design of LuxSit sPro minimizes steric hindrance and preserves native binding interactions.
High Sensitivity and Speed: Under the ideal conditions the pairs can achieve similar performance to full ELISA protocols in just 15-minutes
LuxLink Kit Components
| LuxLink Antibody Conjugation System Component Name | Unit Size |
|---|---|
| βit-TFP Ester (10 mM in DMSO) | 1 x 10 µL |
| Tetrazine-αLux | 2 x 100 µg |
| NHS-TCO Linker (2 mM in DMSO) | 1 x 10 µL |
The LuxLink Antibody Conjugation System kit provides reagents for the conjugation of up to 200 µg of antibody to both the βit and αLux fragments.
LuxLink Conjugation Kit Yields Highly Sensitive Assay Performance in Varied Matrices
